Development and validation of a new method to isolate, expand, and differentiate circulating osteogenic precursor (COP) cells

Circulating osteogenic precursor (COP) cells are a population of progenitor in the peripheral blood with capacity to form bone vitro and vivo . They have characteristics mesenchymal stem pool found marrow; however, more recently, COP has been identified markers hematopoietic lineage such as CD45 CD34. While this associated several pathologies, lack cell culture models inconsistent characterization limited mechanistic research into their behavior physiology. In study, we describe method for isolation CD45+/CD34+/alkaline phosphatase (ALP) + via fluorescence-activated sorting, well expansion differentiation culture. Hematopoietic discreet within monocyte fraction mononuclear cells, which proliferative, fibroblastoid colonies Their expression decreases time culture, but they express osteogenesis deposit calcium differentiation. It is hoped that will provide standard isolation, consistency future efforts, allow translation clinical settings. • A research. We developed validated new isolate, expand, differentiate cells.